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By B.T. Kang, A.N. Atta-Krah, L. Reynolds

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Tannic add is jtn example of a mordant. They are ' Simple Sftains. Many different kinds of staining solutions are Some are for general emplt>yeffin the various bacteriological procedures. BIOLOGICAL STAINS 41 use and others are adapted to specific purposes. A simple staining solution is one that contains only a single dye dissolved in the solvent. The simple staining It is applied to the bacteria in one application. solutions that are employed probably more than any of the others for routine purposes are dilute carbolfuchsin, methylene blue, and gentian violet or crystal violet.

Light (obtained Lens systems corrected for light of three different wave lengths are apochromatic objectives. These objectives are composed of fluorite in combination with lenses of optical glass. The images produced called in this group exhibit only a faint blue or yellow residual Since these objectives are corrected for three colors instead of for two, they are superior to the achromats. Their finer color correction by objectives color. makes The violet rays possible a greater usable numerical aperture.

Is also a member of compounds. Chemically triphenyl-methane group and the formula is as follows: of the triamino- it is hexamethyl p-rosaniline CH 3 Crystal violet The dye known violet 10 J5, gentian violet, hexa the methyl deepest shade of the p-rosanilines produces and is considered the most satisfactory of all the violet compounds as a also is violet, etc. as methyl It simple stain for general purposes. Differential stains are composed of more than In some of the staining techniques, the dyes are applied The separately; in others they are mixed and applied in one solution.

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